from __future__ import division, print_function, absolute_import import os import warnings import numpy as np from numpy.testing import (assert_equal, run_module_suite, assert_raises, assert_) import scipy.ndimage as ndi import skimage from skimage import io, draw, data_dir from skimage.data import binary_blobs from skimage.util import img_as_ubyte from skimage.morphology import skeletonize_3d # basic behavior tests (mostly copied over from 2D skeletonize) def test_skeletonize_wrong_dim(): im = np.zeros(5, dtype=np.uint8) assert_raises(ValueError, skeletonize_3d, im) im = np.zeros((5, 5, 5, 5), dtype=np.uint8) assert_raises(ValueError, skeletonize_3d, im) def test_skeletonize_1D(): # a corner case of an image of a shape(1, N) im = np.ones((5, 1), dtype=np.uint8) res = skeletonize_3d(im) assert_equal(res, im) def test_skeletonize_no_foreground(): im = np.zeros((5, 5), dtype=np.uint8) result = skeletonize_3d(im) assert_equal(result, im) def test_skeletonize_all_foreground(): im = np.ones((3, 4), dtype=np.uint8) assert_equal(skeletonize_3d(im), np.array([[0, 0, 0, 0], [1, 1, 1, 1], [0, 0, 0, 0]], dtype=np.uint8)) def test_skeletonize_single_point(): im = np.zeros((5, 5), dtype=np.uint8) im[3, 3] = 1 result = skeletonize_3d(im) assert_equal(result, im) def test_skeletonize_already_thinned(): im = np.zeros((5, 5), dtype=np.uint8) im[3, 1:-1] = 1 im[2, -1] = 1 im[4, 0] = 1 result = skeletonize_3d(im) assert_equal(result, im) def test_dtype_conv(): # check that the operation does the right thing with floats etc # also check non-contiguous input img = np.random.random((16, 16))[::2, ::2] img[img < 0.5] = 0 orig = img.copy() with warnings.catch_warnings(): # UserWarning for possible precision loss, expected warnings.simplefilter('ignore', UserWarning) res = skeletonize_3d(img) assert_equal(res.dtype, np.uint8) assert_equal(img, orig) # operation does not clobber the original assert_equal(res.max(), img_as_ubyte(img).max()) # the intensity range is preserved def test_input(): # check that the input is not clobbered # for 2D and 3D images of varying dtypes imgs = [np.ones((8, 8), dtype=float), np.ones((4, 8, 8), dtype=float), np.ones((8, 8), dtype=np.uint8), np.ones((4, 8, 8), dtype=np.uint8), np.ones((8, 8), dtype=bool), np.ones((4, 8, 8), dtype=bool)] for img in imgs: yield check_input, img def check_input(img): orig = img.copy() with warnings.catch_warnings(): # UserWarning for possible precision loss, expected warnings.simplefilter('ignore', UserWarning) res = skeletonize_3d(img) assert_equal(img, orig) def test_skeletonize_num_neighbours(): # an empty image image = np.zeros((300, 300)) # foreground object 1 image[10:-10, 10:100] = 1 image[-100:-10, 10:-10] = 1 image[10:-10, -100:-10] = 1 # foreground object 2 rs, cs = draw.line(250, 150, 10, 280) for i in range(10): image[rs + i, cs] = 1 rs, cs = draw.line(10, 150, 250, 280) for i in range(20): image[rs + i, cs] = 1 # foreground object 3 ir, ic = np.indices(image.shape) circle1 = (ic - 135)**2 + (ir - 150)**2 < 30**2 circle2 = (ic - 135)**2 + (ir - 150)**2 < 20**2 image[circle1] = 1 image[circle2] = 0 result = skeletonize_3d(image) # there should never be a 2x2 block of foreground pixels in a skeleton mask = np.array([[1, 1], [1, 1]], np.uint8) blocks = ndi.correlate(result, mask, mode='constant') assert_(not np.any(blocks == 4)) def test_two_hole_image(): # test a simple 2D image against FIJI img_o = np.array([[0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0], [0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 1, 0, 0, 0, 0, 0], [0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0, 1, 0], [0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], [0, 1, 1, 1, 1, 0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0], [0, 1, 1, 1, 0, 0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0], [0, 0, 1, 1, 0, 0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0], [0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0], [0, 0, 0, 1, 1, 0, 0, 1, 1, 1, 0, 0, 1, 1, 1, 0], [0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], [0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], [0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 0], [0, 0, 0, 0, 0, 0, 1, 1, 1, 1, 1, 1, 1, 0, 0, 0], [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0]], dtype=np.uint8) img_f = np.array([[0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], [0, 0, 0, 0, 0, 0, 1, 1, 0, 1, 1, 0, 0, 0, 0, 0], [0, 0, 0, 0, 1, 1, 0, 0, 1, 0, 0, 1, 0, 0, 0, 0], [0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 1, 0, 0, 0], [0, 0, 1, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0], [0, 0, 1, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0], [0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0], [0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0], [0, 0, 0, 0, 1, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0], [0, 0, 0, 0, 0, 1, 1, 0, 1, 0, 0, 0, 1, 0, 0, 0], [0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 1, 1, 0, 0, 0, 0], [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0]], dtype=np.uint8) res = skeletonize_3d(img_o) assert_equal(res, img_f) def test_3d_vs_fiji(): # generate an image with blobs and compate its skeleton to # the skeleton generated by FIJI img = binary_blobs(32, 0.05, n_dim=3, seed=1234) img = img[:-2, ...] img = img.astype(np.uint8)*255 img_s = skeletonize_3d(img) img_f = io.imread(os.path.join(data_dir, "_blobs_3d_fiji_skeleton.tif")) assert_equal(img_s, img_f) if __name__ == '__main__': run_module_suite()